Spain's Villa sidelined by arthritic ankle
Atletico Madrid's forward David Villa dribbles the ball during a training session at the Dragao stadium in Porto on September 30, 2013
The Atletico Madrid striker was forced off just before the hour mark of his side's 2-1 win over Celta Vigo on Sunday.
"The Asturian player is suffering from arthritis in his left ankle, the medical services of the Spanish Football Federation (RFEF) have been informed, which will make his presence in the squad managed by Vicente del Bosque impossible," the RFEF said in a statement.
Villa has featured in every one of Atletico's 12 games in all competitions this season, scoring three goals as Diego Simeone's men have made a flying start to the campaign.
And Simeone is hopeful Villa will be fit for Atletico's next match against Espanyol in two weeks' time.
"He went off with some problems in his ankle. I imagine that in a short while it will respond and he will be fine," he said.
Del Bosque has already had to call up Real Sociedad's Inigo Martinez to replace the injured Raul Albiol.
And it is expected that Del Bosque will add another striker to the squad with Tottenham Hotspur's Roberto Soldado the favourite to replace the 31-year-old Villa.
Spain need just four points from their remaining two qualifiers to guarantee their place at next year's World Cup finals in Brazil.